103680 Demethylation and auto-oxidation of different cobalt-bleomycin complexes

Contact an Account Manager

Demethylation and auto-oxidation of different cobalt-bleomycin complexes

Summary

Type: HPLC
Application #: 103680
Catalog#: 720023.40 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4x250mm
Detection: UV, 254nm
Conditions: eluent: 1% ammonium acetate - methanol (6:4, v/v)
flow rate 2ml/min
Authors: Vos CM
Source: J Antibiotics 35 (1982) 837 - 842


Details


Legend: Fig. . Chromatograms of Co-bleomycin-demethyl A2 complexes a) Co-blm A2, demethylated by heating at 120°C overnight b) 0.7mM blm dA2, chelated with cobalt, chromatographed immediately after preparat