103560 Determination of amoxicillin in plasma by HPLC with fluorescence detection after on-line oxidation

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Determination of amoxicillin in plasma by HPLC with fluorescence detection after on-line oxidation

Summary

Type: HPLC
Application #: 103560
Detection: fluorescence, excitation 255nm, emission 400nm after postcolumn electrochemical oxidation at 0.78V
Conditions: eluent: 0.02M methanesulphonic acid - acetonitrile (92.5:7.5, v/v)
Authors: Mascher, H.J. et al.
Source: J. Chromatogr. 506 (1990) 417–421


Details


Legend: Fig. 2. Chromatograms obtained after injection of 20µl deproteinized plasma, (a) pre-dose plasma, (b) plasma spiked with 0.42µg/ml amoxicillin (tR 2.1min), (c) plasma from a volunteer after a single