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Simplified HPLC method for 5-aminosalicylic acid in plasma and urine
Summary
Type: HPLCApplication #: 103330
Catalog#: 720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection: fluorescence, excitation 300nm, emission 418nm
Conditions: eluent: deionized water adjusted to pH 3 with conc. perchloric acid - methanol - acetonitrile (75:12.5:12.5)
flow rate 0.5 - 0.6ml/min
pressure about 300bar
Authors: Fischer C. et al.
Source: J. Chromatogr. 225 (1981) 498–503
Details
Legend: Fig. 1. HPLC separation of a) 50ng each of 5AS, 5AcAS and AcPAS, injected directly into the HPLC system, b) extract of 500µl blank plasma spiked with internal standard, c) and d) extracts of 500µl b