103250 Determination of minor impurities in temafloxacin hydrochloride by HPLC

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Determination of minor impurities in temafloxacin hydrochloride by HPLC

Summary

Type: HPLC
Application #: 103250
Catalog#: 720002.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x125mm
Detection: UV, 325nm, 0.10AUFS
Conditions: eluent A: 0.02M KH2PO4 adjusted to pH 2.4 with H3PO4
eluent B: acetonitrile
eluent C: tetrahydrofuran
gradient A : B : C = 90 : 5 : 5 to 35 : 5 : 60 in 50min, hold 10min, then reequilibration to 90:5:5 in 5min, hold 15min
flow rate 1ml/min
Authors: Elrod L Jr
Source: J Chromatogr 519 (1990) 125 - 136


Details


Legend: Fig. 3. Chromatograms for temafloxacin hydrochloride (tema) and impurities, A) mixed standard preparation, B) temafloxacin hydrochloride (lot 1) spiked with known impurities at approx. 0.5 to 1.2%,