103230 Determination of ofloxacin in biological fluids using HPLC with fluorimetric detection

Contact an Account Manager

Determination of ofloxacin in biological fluids using HPLC with fluorimetric detection

Summary

Type: HPLC
Application #: 103230
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: fluorescence, excitation 310nm, emission 487nm
Conditions: eluent: acetonitrile - 50mM phosphate buffer pH 2.8 containing 6mmol/l triethylamine (18:82, v/v)
flow rate 1.0ml/min
Authors: Kraas E
Source: Z Anal Chem 324 (1986) 354


Details


Legend: Fig. 1. HPLC chromatograms from human serum, a) serum with 5.53µmol/l (2.0mg/l) ofloxacin, b) blank serum.