103220 Improved HPLC determination of pefloxacin and its metabolite norfloxacin in human plasma and tissue

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Improved HPLC determination of pefloxacin and its metabolite norfloxacin in human plasma and tissue

Summary

Type: HPLC
Application #: 103220
Detection: fluorescence, excitation 330nm, emission 440nm
Conditions: eluent: 150ml acetonitrile, 2g Na acetate trihydrate, 2g citric acid monohydrate, 1ml triethylamine, 850ml water, pH 4.8
flow rate 2.0ml/min
Authors: Montay, G.
Source: J Chromatogr 339 (1985) 214–218


Details


Legend: Fig. 2. Typical HPLC profiles of prostatic tissue extracts: A) blank, B) 0.025µg norfloxacin and 0.25µg pefloxacin standards, C) 0.30µg norfloxacin and 3µg pefloxacin standards, D) tissue extract of