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Assay of ciprofloxacin and norfloxacin in serum and urine by HPLC
Summary
Type: HPLCApplication #: 103200
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: fluorescence, excitation 278nm, emission 445nm
Conditions: eluent: acetonitrile - 0.025M o-phosphoric acid adjusted to pH 3.0 with tetrabutylammonium hydroxide (11:89, v/v)
flow rate 1.5ml/min
Authors: Nilsson-Ehle I
Source: J Chromatogr 416 (1987) 207 - 211
Details
Legend: Fig. 1. Chromatograms of samples obtained from a patient receiving 400mg norfloxacin orally, a) serum sample, 2.58µg/ml, 4h after administration, b) serum blank, c) urine sample, 48.4µg/ml, 5h after