103160 Assay of trimethoprim in plasma and urine by HPLC using electrochemical detection

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Assay of trimethoprim in plasma and urine by HPLC using electrochemical detection

Summary

Type: HPLC
Application #: 103160
Catalog#: 720002.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x125mm
Detection: UV, 280 or electrochemical detection at 1200 mV
Conditions: eluent: 0.07M KH2PO4 pH 4.75 - methanol (3:1)
flow rate 1.5ml/min
pressure 200bar
Authors: Nordholm L
Source: J Chromatogr 233 (1982) 427 - 431


Details


Legend: Fig. 2. Electrochemical detection at 1200mV (top) and UV detection at 280nm (bottom) of A) blank plasma extract, B) plasma extract from a 67-day-old pig 7h after intravenous injection of 5mg/kg TMP.