102920 Determination of the g-aminobutyric acid agonist 4,5,6,7-tetrahydroisooxazolo[5,4-c]pyridin-3-ol by HPLC using UV or electrochemical detection

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Determination of the g-aminobutyric acid agonist 4,5,6,7-tetrahydroisooxazolo[5,4-c]pyridin-3-ol by HPLC using UV or electrochemical detection

Summary

Type: HPLC
Application #: 102920
Catalog#: 720002.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x125mm
Detection: in series: UV, 217nm, 0.1 AUFS (left hand trace) and electrochemical, potential +0.98V (right hand trace)
Conditions: eluent: 0.02M Na acetate pH 4.5 - tetrahydrofuran (90:10) with 5mmol/l perfluorooctanesulphonic acid and 50mmol/l lithium perchlorate
flow rate 2.0ml/min
Authors: Madsen SM
Source: J Chromatogr 238 (1982) 509 - 514


Details


Legend: Fig. 1. Chromatogram of a sample containing THIP formed by hydrolysis of a derivative from THIP.