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Reversed phase ion pair HPLC study of myoinositol phosphates
Summary
Type: HPLCApplication #: 102810
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: upper trace UV, 254nm, lower trace radioactivity, peak 1: UV at 340nm after enzymatic reaction of corresponding fractions, peak 3 UV at 240nm after enzymatic reaction of fractions
Conditions: eluent A: 10mM tetrabutylammonium hydrogen sulphate (TBAHS), 15mM KH2PO4, pH 5.0
eluent B: 10mM TBAHS, 35mM KH2PO4, 30% acetonitrile, pH of the aqueous part 5.0
gradient 12min 1% B, 1 - 31% B in 3min, 31 - 55% B in 3min, hold 15min, 55 - 85% B in 3min, hold 6min (see dotted line)
flow rate 1ml/min
Authors: Patthy M
Source: J Chromatogr 523 (1990) 201 - 216
Details
Legend: Fig. 9. Separation of myo-inositol phosphates, some common nucleotides and sugar phosphates, 1. glucose-6-phosphate, 2. inositol 1-phosphate, 3. D-fructose-1,6-diphosphate, 4. and 6. unknown, 5. ino