102780 Reversed phase ion pair LC of glucuronides

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Reversed phase ion pair LC of glucuronides

Summary

Type: HPLC
Application #: 102780
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV, 254nm
Conditions: eluent: 0.05M glycine buffer pH 3.0, 50% (v/v) methanol, 20.0mM dodecylamine·HCl
flow rate 1ml/min
Authors: Stefansson M
Source: J Chromatogr 499 (1990) 411 - 421


Details


Legend: Fig. 1. Chromatogram of 20µl spiked urine containing 12 - 40nmol of each glucuronide 1.) 2-aminophenyl-b-D-glucuronide 2.) 8-hydroxyquinoline-b-D-glucuronide 3.) 4-nitrophenyl-b-D-glucuronide 4.