102750 HPLC analysis of uronic acids and oligogalacturonic acids

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HPLC analysis of uronic acids and oligogalacturonic acids

Summary

Type: HPLC
Application #: 102750
Catalog#: 720029.46 - MN HPLC Column, Nucleosil SB, 100A, 10um, 4.6x250mm
Detection: UV, 235nm
Conditions: eluent: Na acetate buffer pH 5.4, for a) 0.3M, for b) 0.4M
flow rate 1ml/min
column temperature 40°C
Authors: Voragen AGJ
Source: J Chromatogr 244 (1982) 327 - 336


Details


Legend: Fig. 3. Separation of unsaturated oligogalacturonic acids, U-di = unsaturated digalacturonic acid, U-tri = unsaturated trigalacturonic acid etc. a) Separation of oligomers up to hexamers, b) separat