102670 Separation of glucosinolates by HPLC

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Separation of glucosinolates by HPLC

Summary

Type: HPLC
Application #: 102670
Catalog#: 720002.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x125mm
Detection: UV, 235nm
Conditions: solvent velocity: 1.5mm/sec
Eluent for fig. 1 (left): 0.01M phosphate buffer pH 7.0 - methanol (95:5)
Eluent for fig. 4 (right): 0.01M phosphate buffer pH 7.0 - methanol (3:7) containing 0.005M tetraoctylammonium bromide
Authors: Helboe P
Source: J Chromatogr 197 (1980) 199 - 205


Details


Legend: Chromatograms of glucosinolates 2. sinigrin (allyl glucosinolate), 11. glucotropaeolin (benzyl glucosinolate), 13. sinalbin (p-hydroxybenzyl glucosinolate), and 16. glucobrassicin (indol-3-ylmethyl