102490 Determination of monosaccharides constituting the glycosides in saponins by HPLC

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Determination of monosaccharides constituting the glycosides in saponins by HPLC

Summary

Type: HPLC
Application #: 102490
Catalog#: 720095.46 - MN HPLC Column, Nucleosil NH2, 100A, 5um, 4.6x250mm
Detection: tetrazolium blue (TB) solution for postcolumn derivatization: 2% (w/v) TB in 0.2M aq. NaOH - ethanol (1:1), flow rate 0.25ml/min; detection: UV-VIS, 546nm
Conditions: eluent: acetonitrile - water (8:2)
flow rate 1.5ml/min
Authors: Kikuchi J
Source: J Chromatogr 403 (1987) 319 - 323


Details


Legend: Fig. 1. Chromatogram of 1. rhamnose, 2. xylose, 3. arabinose, 4. glucose, 5. galactose, 6. maltose (int. std.).