102350 Detection by HPLC of polyamines formed by clostridial putrefaction of caseins

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Detection by HPLC of polyamines formed by clostridial putrefaction of caseins

Summary

Type: HPLC
Application #: 102350
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: fluorescence
Conditions: eluent A: methanol - 0.08 M acetic acid - acetonitrile (45:40:15)
eluent B: 100% methanol
15min isocratic eluent A, then linear gradient to 100% B in 10min, finally 20min isocratic eluent B
Authors: Karlsson S
Source: J Chromatogr 442 (1988) 267 - 277


Details


Legend: Fig. 1. HPLC trace of casein degraded by Clostridium sporogenes at 20°C, pH 7.5. 1.) Histamine 2). Agmatine 3.) Serotonin 4.) Tyramine 5.) Tryptamine 6.) Putrescine 7.) Cadaverine