102290 Electrochemical Determination of Histamine Derivatised with O-Phthalaldehyde and 2-Mercaptoethanol

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Electrochemical Determination of Histamine Derivatised with O-Phthalaldehyde and 2-Mercaptoethanol

Summary

Type: HPLC
Application #: 102290
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: electrochemical, glassy carbon electrode, +0.5V versus Ag/AgCl
Conditions: eluent: 0.07M Na2HPO4 - citric acid buffer, pH 4.5,
containing 23% acetonitrile and 0.26mM Na2EDTA
flow rate 1ml/min
Authors: Harsing LG
Source: J Chromatogr 383 (1986) 19 - 26


Details


Legend: Fig. 1. HPLC profiles of histamine derivatized with OPA (left) and OPA/2-ME (right). A small peak with a retention time of 10.5min also appeared in the HPLC profile of histamine derivatized with OPA/2