102280 Separation of aromatic choline esters by HPLC

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Separation of aromatic choline esters by HPLC

Summary

Type: HPLC
Application #: 102280
Catalog#: 720001.46 - MN HPLC Column, Nucleosil C8, 100A, 5um, 4.6x125mm
Detection: UV, 280nm
Conditions: eluent A:
0.02M phosphate buffer, 0.02M dibutylamine and 0.02M Na heptanesulphonic acid, pH 2.0 - acetonitrile (1:1),
eluent B:
0.01M phosphate buffer, 0.01M dibutylamine and 0.01M Na heptanesulphonic acid, pH 2.0

linear gradient from A:B = 2:8 to 7:3 in 30min
flow rate 1ml/min
Authors: Clausen S
Source: J Chromatogr 260 (1983) 193 - 199


Details


Legend: Fig. 1. Chromatogram of aromatic choline esters benzoylcholine derivatives: 1.) 4-hydroxybenzoylcholine 4.) 2,3-dihydroxybenzoylcholine 7.) 3,5-dihydroxybenzoylcholine 9.) 3-hydroxy-4-methoxybe