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Histamine assay in tears by fluorescamine derivatization and HPLC
Summary
Type: HPLCApplication #: 102270
Catalog#: 720022.40 - MN HPLC Column, Nucleosil C8, 100A, 10um, 4x250mm
Detection: fluorescence: excitation 390nm, emission 480nm
Conditions: eluent: 0.004M phosphate buffer adjusted to pH 3.5 - acetonitrile (35:65),
flow rate 0.5ml/min,
injection volume 20μl.
Authors: Bettero A
Source: J Chromatogr 310 (1984) 390 - 395
Details
Legend: Chromatograms of histamine (Hm) from standard solution (20ng/ml) and from normal (5.2ng/ml) and pathological (50.6ng/ml) human tear samples