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Determination of acetylcholine and choline in rat brain
Summary
Type: HPLCApplication #: 102240
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: electrochemical: +500mV versus Ag/AgCl
Conditions: eluent: 0.091M Na acetate pH 5.0, 25mg/l Na octyl sulphonate, 1.2mM tetramethylammonium chloride, 10mM Na2EDTA, flow rate 0.8ml/min, temperature 37°C.
Authors: Asano, M.
Source: J Liquid Chromatogr 9 (1986) 199 - 215
Details
Legend: Chromatogram of a standard mixture. Ch = choline, EHC = ethylhomocholine (int. std.), ACh = acetylcholine, 200pmol each injected.