102220 Determination of 8-methyl ether of xanthurenic acid in human urine by HPLC

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Determination of 8-methyl ether of xanthurenic acid in human urine by HPLC

Summary

Type: HPLC
Application #: 102220
Catalog#: 720120.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x150mm
Detection: fluorescence: exitation 340 nm, emission 450 nm
Conditions: eluent: 0.03 M KH2PO4 (pH 6.0) : acetonitrile (1000 : 140, v/v) containing 0.1 mM Na2EDTA,
flow rate 0.7 ml/min,
column temperature 40 °C.
Authors: Imai J
Source: J Chromatogr B 661 (1994) 149 - 153


Details


Legend: HPLC chromatograms for urinary XA-OMe. (A) XA-OMe standard (0.1022 µg/ml). (B) normal adult urine processed by the direct dilution method (B-1, 0.0107 µg/ml, diluted ten-fold) and by the extraction