102172 Gradient elution of biogenic amines and derivatives (B)

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Gradient elution of biogenic amines and derivatives (B)

Summary

Type: HPLC
Application #: 102172
Catalog#: 720013.40 - MN HPLC Column, Nucleosil C8, 100A, 5um, 4x250mm
Detection: coulometric: +700mV
Conditions: eluent A: 0.20M perchlorate pH 2.15,
eluent B: 0.02M perchlorate pH 6.00,
gradient: 0 - 99% B in 9min, linear,
flow rate 1.5ml/min,
temperature 300K
Authors: Tjaden UR
Source: J Liquid Chromatogr 6 (1983) 2255 - 2274


Details


Legend: Gradient elution of the test mixture (180 - 520pmol per compound injected): 3-methoxy-4-hydroxyphenylethylene glycol, epinephrine, norepinephrine, dopa, vanillylmandelic acid, dopamine, tyros