102140 Simultaneous measurement of L-dopa its metabolites and carbidopa in plasma of Parkinsonian patients

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Simultaneous measurement of L-dopa its metabolites and carbidopa in plasma of Parkinsonian patients

Summary

Type: HPLC
Application #: 102140
Catalog#: 720002.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x125mm
Detection: coulometric: E1 + 0.2V, E2 -0.35V
Conditions: eluent: 0.013M Na acetate containing 100mg//l Na octanesulphonate, 200mg/l Na2EDTA and 15% methanol pH 2.82,
flow rate 0.8ml/min
Authors: Lucarelli C
Source: J Chromatogr 511 (1990) 167 - 176


Details


Legend: A) 10µl standard solution, B) 20µl deproteinized plasma sample, C) sample as in B) diluted 20fold. L-dopa, 3,4-dihydroxyphenylacetic acid, dopamine, N-methyldopamine (int. std.), 3-O-methyldo