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Automatic liquid chromatography of urinary dopamine
Summary
Type: HPLCApplication #: 102060
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: fluorescence: excitation 282nm, emission 315nm
Conditions: eluent: 0.003M octyl sulphate, 0.035M KH2PO4, 0.03M citric acid, 0.001M ascorbic acid and 14% (v/v) methanol,
flow rate 2ml/min,
temperature 20°C
Authors: Guyon, F. et al.
Source: J. Chromatogr. 311 (1984) 160–166
Details
Legend: A) Ion pair separation of a dopamine standard solution (0.2mg/l = 1.3µmol/l, DHBA = int. std.) after enrichment on an alumina precolumn. B) Urine sample analysed under the same conditions as in (A