102030 A highly sensitive assay for nonspecific N-methyltransferase activity in rat tissues

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A highly sensitive assay for nonspecific N-methyltransferase activity in rat tissues

Summary

Type: HPLC
Application #: 102030
Catalog#: 720018.40 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4x250mm
Detection: electrochemical: potential +0.6V versus Ag/AgCl
Conditions: eluent: 0.1M Na phosphate buffer pH 2.6, 5mM Na n-pentanesulphonate, 0.25% (v/v) acetonitrile,
flow rate 0.9ml/min,
temperature: 25 - 28°C,
injection volume 50μl
Authors: Boonyarat D
Source: J Chromatogr 274 (1983) 331 - 336


Details


Legend: Elution patterns of nonspecific N-methyltransferase incubation mixtures with homogenizate of rat whole brain as enzyme, 20µM dopamine (DA) and 24µM S-adenosyl-L-methionine (SAM) as substrates, (A