102010 Simple and sensitive assay of dopamine beta-hydroxylase in human cerebrospinal fluid

Contact an Account Manager

Simple and sensitive assay of dopamine beta-hydroxylase in human cerebrospinal fluid

Summary

Type: HPLC
Application #: 102010
Catalog#: 720018.40 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4x250mm
Detection: electrochemical: potential 0.9V versus Ag/AgCl
Conditions: eluent: 0.05M K phosphate buffer pH 7.2 containing 20% methanol,
flow rate 1.0ml/min
Authors: Suzuki H
Source: J Chromatogr 341 (1985) 176 - 181


Details


Legend: Chromatograms using DBH incubation mixture of human child CSF as enzyme. (A) Fusaric acid blank incubation, (B) experimental incubation, (C) 50pmol octopamine added to the fusaric acid blank;