101990 HPLC of O-methyl-catecholamine metabolites as DANS derivatives

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HPLC of O-methyl-catecholamine metabolites as DANS derivatives

Summary

Type: HPLC
Application #: 101990
Catalog#: 720023.40 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4x250mm
Detection: fluorescence
Conditions: A) eluent: ethyl acetate - cyclohexane (75 + 50, v/v), flow rate 0.84ml/min, pressure: 50bar, temperature: 22°C; B) eluent: acetonitrile -water (64 + 36, v/v), flow rate 1.3ml/min, pressure: 6
Authors: Schwedt, G. et al.
Source: Z. Anal. Chem. 285 (1977) 381–384


Details


Legend: Fig. A) Separation of DANS-O-methylcatecholamines by adsorption chromatography, injection volume 25µl (250ng amine each). Fig. B) Separation of DANS-O-methylcatecholamines by reversed phase chrom