101980 Analysis of trace amounts of catecholamines and related compounds in brain tissue

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Analysis of trace amounts of catecholamines and related compounds in brain tissue

Summary

Type: HPLC
Application #: 101980
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: electrochemical: potential 550mV
Conditions: eluent: citrate - phosphate buffer 0.35M, pH 5.8,
flow rate 1.0ml/min,
50μl sample loop
Authors: Westerink B H C
Source: J Liquid Chromatogr 6 (1983) 2337 - 2351


Details


Legend: a) Chromatogram of 64mg hypothalamic tissue (50% of the sample), b) chromatogram of the remaining 50% of the same sample after thermic hydrolysis (10min, 100°C), c) chromatogram of 40mg hypothalami