101910 Fully automated HPLC assay for the analysis of free catecholamines in urine
Home sorbtech.com Back-
Contact an Account Manager
Fully automated HPLC assay for the analysis of free catecholamines in urine
Summary
Type: HPLCApplication #: 101910
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: fluorescence: excitation 280nm, emission 310nm
Conditions: eluent: methanol - 0.075M NaH2PO4·H2O, 0.00015M EDTA, 0.006M Na heptanesulphonate pH 3.96 (18:32, v/v), flow rate 1.25ml/min
Authors: Said R
Source: J Chromatogr 530 (1990) 11 - 18
Details
Legend: Chromatograms of catecholamines: c) urinary pool spiked with 0.5µM E, NE and D, d) urinary pool spiked with 1.0µM E, NE and D, IS = dihydrobenzylamide hydrobromide (DHBA, int. std.).