101900 Analysis of biogenic amines and their acidic metabolites

Contact an Account Manager

Analysis of biogenic amines and their acidic metabolites

Summary

Type: HPLC
Application #: 101900
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: electrochemical: glassy carbon electrode at 0.75V versus Ag/AgCl
Conditions: eluent A: 500ml aqueous solution of 0.05g Na dodecyl sulphate (SDS), 0.05g NaCl and 0.05g EDTA and 88ml acetonitrile,
eluent B: 0.05g SDS, 0.05G NaCl, 0.05g EDTA, 0.10g Na octanesulphonate and 214ml acetonitrile,
both adjusted to pH 3.3 with 0.15M H3PO4,
step gradient: system equilibration with eluent A for 30 - 40min, then switched to eluent B before sample injection,
flow rate 1ml/min
Authors: Yi Z
Source: J Liquid Chromatogr 13 (1990) 2161 - 2177


Details


Legend: Chromatogram of a standard mixture of vanillylmandelic acid (VMA), 3-methoxy-4-hydroxyphenylethylene glycol (MHPG), 3,4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxyindoleacetic acid (5HIAA), h