101890 Derivatization of catecholamines and amphetamine with 9-fluorenylmethyl chloroformate for HPLC

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Derivatization of catecholamines and amphetamine with 9-fluorenylmethyl chloroformate for HPLC

Summary

Type: HPLC
Application #: 101890
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV 265nm
Conditions: eluent: acetonitrile - water (58:42, v/v),
flow rate 1.5ml/min
Authors: Maeder, G. et al.
Source: J. Chromatogr. 593 (1992) 9–14


Details


Legend: Chromatogram obtained for a mixture of derivatized amphetamines (off-line method), amounts injected 2·10-10 mol for each compound: FMOC-OH (3.50 min.), norephedrine (6.40), ephedrine (7.65), F