101670 Gradient elution of 16 PAHs with fluorescence detection

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Gradient elution of 16 PAHs with fluorescence detection

Summary

Type: HPLC
Application #: 101670
Catalog#: 720923.40 - MN HPLC Column, Nucleosil C18 PAH, 100A, 5um, 4x150mm
Detection: fluorescence, wavelength program
Conditions: eluent: water - acetonitrile, 5 min 60% (v/v) ACN, isocratic, then linear gradient, 60 - 90% ACN in 15min, 90 - 100% ACN in 20min, 20min 100% ACN, isocratic; reequilibration 10min under initial conditions,
flow rate 0.9ml/min,
temperature: 20°C,
pressure: 1500psi
Authors: Macherey-Nagel
Source: LC department


Details


Legend: naphthalene (1.0ng), acenaphthene (0.4ng), fluorene (1.1ng), phenanthrene (0.5ng), anthracene (1.4ng), fluoranthene (1.4ng), pyrene (1.3ng), benz[a]anthracene (1.3ng), chrysene (1.8ng), benzo