101610 Determination of sulphonated azo dyestuffs and their bacterial metabolites by HPLC

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Determination of sulphonated azo dyestuffs and their bacterial metabolites by HPLC

Summary

Type: HPLC
Application #: 101610
Catalog#: 720018.46 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4.6x250mm
Detection: UV 220nm
Conditions: eluent A: 100mM K phosphate buffer, pH 6.7,
eluent B: methanol - 10mM K phosphate,
pH 6.7 (7:3 v/v),
10min eluent A, then linear gradient from 0 to 100% B in 30min,
flow rate 0.7ml/min
Authors: Grossenbacher H
Source: J Chromatogr 360 (1986) 219 - 223


Details


Legend: Chromatogram of a mixture of sulphonates, I. sulphanilic acid, II. p-hydroxybenzene sulphonic acid, III. metanilic acid, IV. orthanilic acid, V. sulphanilamide, VI. laurent acid, VII. gamma a