101210 Determination of codeine and its metabolites in human blood plasma and in microsomal incubates

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Determination of codeine and its metabolites in human blood plasma and in microsomal incubates

Summary

Type: HPLC
Application #: 101210
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV 214nm
Conditions: eluent: 7% (v/v) acetonitrile - 10mM phosphate buffer pH 2.1 - 2mM propyl sulphate
Authors: Persson K
Source: J Chromatogr 491 (1989) 473 - 480


Details


Legend: Chromatogram of (left) blank and (right) plasma sample spiked with 25ng/ml morphine, 25ng/ml norcodeine (NC), and 50ng/ml codeine (COD), sample extracted with dichloromethane containing 15% 2-propan