101200 Separation of 8 alkaloids and meconic acid and quantitation of 5 principal alkaloids in gum opium

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Separation of 8 alkaloids and meconic acid and quantitation of 5 principal alkaloids in gum opium

Summary

Type: HPLC
Application #: 101200
Catalog#: 720019.40 - MN HPLC Column, Nucleosil Phenyl, 100A, 7um, 4x250mm
Detection: UV 280nm
Conditions: eluent A: methanol - water (5:95), pH* (apparent pH) 3.2, eluent B: methanol - water (80:30), pH* 3.95, 1% (v/v) of stock buffer solution (triethylammonium phosphate) in each, linear gradient from 1
Authors: Ayyangar N R
Source: J Chromatogr 436 (1988) 455 - 465


Details


Legend: HPLC separation of an artificial mixture of nine gum opium constituents: morphine, meconic acid, codeine, thebaine, laudanosine, cryptopine, noscapine, narceine, papaverine.