101090 Quantitation of complex pharmaceutical raw materials

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Quantitation of complex pharmaceutical raw materials

Summary

Type: HPLC
Application #: 101090
Detection: UV 254nm
Conditions: eluent: acetonitrile - 0.01M (NH4)2CO3 in water,
step1: 8% before injection,
step2: 15%, 1min,
step3: 30%, 1min,
step4: 40%, 3min,
step5: 50%, 2min,
step6: 60%, 4min,

flow rate 1.5ml/min,
pressure approx. 350bar,
injection volume 30μl.
Authors: Erni, F.
Source: J. Chromatogr. 125 (1976) 265–274


Details


Legend: Separation of ergot alkaloids, 1. lysergic acid, 2. isolysergic acid, 3. lysergic acid amide, 4. isolysergic acid amide, 5. aci-ergotamine, 6. aci-ergotaminine, 7. ergotamine, 8. ergotaminine;