100890 Determination of benzene metabolites in urine of mice by solid phase extraction and HPLC

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Determination of benzene metabolites in urine of mice by solid phase extraction and HPLC

Summary

Type: HPLC
Application #: 100890
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV 270nm
Conditions: eluent: phosphate solution pH 3.4 - methanol (70:30, v/v),
flow rate 1ml/min;
trans,trans-muconic acid could be quantitated in fraction II using the same HPLC conditions.
Authors: Schad, H. et al.
Source: J. Chromatogr. 593 (1992) 147–151


Details


Legend: Chromatograms of urine from mice exposed to 300ppm benzene for 6h a day, 5days per week, A) fraction I after solid phase extraction, B) fraction I after evaporation.