100650 HPLC analysis of phenolic antioxidants, tocopherols and triglycerides

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HPLC analysis of phenolic antioxidants, tocopherols and triglycerides

Summary

Type: HPLC
Application #: 100650
Catalog#: 720051.40 - MN HPLC Column, Nucleosil C18, 120A, 5um, 4x125mm
Detection: UV 280nm
Conditions: eluent A: water + phosphoric acid pH 3,
eluent B: acetonitrile - methanol (7:5),
eluent C: isopropanol,
gradient from 70% A, 30% B to 100% B in 25min, 5 min 100% B, from 100% B to 40% B, 60% C in 15min, 18min at this composition, in 1min to 100% B, in 3min to 70% A, 30% B, 10 min equilibration,
flow rate 1ml/min.
Authors: Andrikopoulos, N.K. et al.
Source: J. Am. Oil Chem. Soc. 68 (1991) 359–364


Details


Legend: HPLC of synthetic antioxidants, tocopherols and triglycerides with conjugated double bonds, PG propyldodecyl gallate, THBP 2,4,5-trihydroxybutyrophenone, TBHQ tert.-butylhydroquinone, NDGA nord