100500 Picomole determination of 2,4-dimethoxyanilides by HPLC with electrochemical detection

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Picomole determination of 2,4-dimethoxyanilides by HPLC with electrochemical detection

Summary

Type: HPLC
Application #: 100500
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: electrochemical, potential +1.10 V versus Ag/AgC
Conditions: eluent: acetonitrile - methanol - water (35:22:43, v/v), containing 0.5 g/l lithium perchlorate, pH adjusted to 4.1 with dilute trifluoroacetic acid,
flow rate 1.0ml/min
Authors: Knospe J
Source: J Chromatogr 442 (1988) 444 - 450


Details


Legend: Chromatogram of 2,4-dimethoxyanilides of prostaglandins, approximately 2.5 ng each. TXB2, PGF2a, PGE2, PGD2, 16,16-dimethyl-PGE2.