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Separation and quantitation of leukotrienes
Summary
Type: HPLCApplication #: 100470
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV 280nm, absorbance ranges: 0 - 7min 0.08 AUFS, 7 - 50min 0.04 AUFS
Conditions: eluent: tetrahydrofuran - methanol - water - acetic acid (25:30:45:0.1, v/v),
pH 5.5 with ammonium hydroxide, aqueous phase with 0.1% EDTA to prevent binding to the column of cations, which could lead to excessive retention of sulphidopeptide leukotrienes,
flow rate: 0.9ml/min
Authors: Verhagen J
Source: Prostaglandins Leukotrienes and Medicine 13 (1984)
Details
Legend: Reversed phase chromatogram of synthetic LTB4, LTC4, LTD4 and the leukotrienes produced by human PMNLs, THETE, LTC4, 11t-LTC4, PGB2 (int. std.), LTD4, 6t-LTB4 + 12epi,6t-LTB4, LTB4, 12epi,6