100300 A rapid method for phospholipid separation by HPLC with light-scattering detection

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A rapid method for phospholipid separation by HPLC with light-scattering detection

Summary

Type: HPLC
Application #: 100300
Detection: light-scattering
Conditions: eluent A: chloroform - trifluoroacetic acid (TFA) (400:5),
eluent B: chloroform - methanol - n-heptane - water - TFA (100:400:50:15:5),
dashed line indicates gradient programme;
flow rate: 1.0ml/min
Authors: Breton, L. et al.
Source: J. Chromatogr. 497 (1989) 243–249


Details


Legend: Separation of natural standard phospholipids phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine.