100240 Rapid HPLC determination of fatty acid profiles of lipids by conversion to their hydroxamic acids

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Rapid HPLC determination of fatty acid profiles of lipids by conversion to their hydroxamic acids

Summary

Type: HPLC
Application #: 100240
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV 213nm
Conditions: eluent: 20mM NaH2PO4 buffer, pH 3 with H3PO4, containing 125µl/l of a 1% (w/w) NaNO3 solution, gradient from 70 to 95% methanol, flow rate: 1ml/min, column temperature 40°C.
Authors: Gutnikov G
Source: J Chromatogr 587 (1991) 292 - 296


Details


Legend: Chromatogram of hydroxamic acid derivatives of a FAME mixture (10 - 30nmol each), capric acid, lauric acid, myristic acid, linolenic acid, palmitoleic acid, linoleic acid, palmitic acid, ol