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Determination of the cytochrome P-450 IV marker
Summary
Type: HPLCApplication #: 100210
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: fluorescence, excitation 340nm, emission 420nm
Conditions: eluent: methanol - water (67:33, v/v),
isocratic for 25min, then 10min linear gradient to 97% methanol, finally 5min at this composition,
flow rate 1ml/min;
column temperature 30°C.
Authors: Dirven, H. A. A. M. et al.
Source: J. Chromatogr. 564 (1991) 266–271
Details
Legend: Chromatograms of diethyl ether extracts of microsomal fractions of rat liver after derivatization with 4-bromomethyl-6,7-dimethoxycoumarin (Br-mdmc), w - 1 = (w - 1)-hydroxylauric acid, w = w-hyd