100130 Simultaneous determination of carboxylic acids and carbonyl compounds in estuaries

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Simultaneous determination of carboxylic acids and carbonyl compounds in estuaries

Summary

Type: HPLC
Application #: 100130
Catalog#: 720041.40 - MN HPLC Column, Nucleosil C18, 120A, 5um, 4x250mm
Detection: UV 360nm
Conditions: eluent A: acetonitrile - methanol - 5mM KH2PO4 buffer (1:1.4:6, v/v),
eluent B: acetonitrile - buffer (3:2 (v/v),
gradient program:
at 0 min eluent B, flow rate 0.6ml/min, at 2min eluent B, 0.6ml/min, at 10min eluent A, 1.25ml/min, at 25min eluent A, 1.25ml/min, at 27min eluent B, 1.25ml/min, at 37min eluent B, 0.6ml/min.
Authors: Edelkraut F. et al.
Source: Chromatographia 30 (1990) 432–435


Details


Legend: Chromatogram from an Elbe estuary sample, DNPH derivatives of 1. oxaloacetic acid, 2. oxoglutaric acid, 3. glyoxylic acid, 4. pyruvic acid, 5. oxobutyric acid, 6. glycolaldehyde, 8. glyoxal, 1